April 14th, 2PM EST (1PM CST)
A low-cost core genome marker platform that works well across the diverse Vitis genus.
rhAmpSeq delivers 2000 markers from 22,000 individuals, 91% transferable, covering 97% of the core grape genome should be broadly applicable in other organisms.
Dr. Qi Sun, Senior Research Associate, Cornell Bioinformatics Service Unit, Ithaca, NY
Dr. Cheng Zou, Postdoctoral Associate, Cornell University Institute of Biotechnology, Ithaca, NY
Dr. Avinash Karn, Postdoctoral Associate, School Integrative Plant Science, Horticulture Section, Cornell AgriTech, Geneva, NY
Several considerations determine whether a DNA marker platform will be widely used, including cost, throughput, genome coverage, information content, simple interpretation, and transferability across diverse taxa and programs. The VitisGen2 project has a marker strategy called ‘rhAmpSeq’ that meets these requirements and should be of broad interest to biologists.
To develop it, we first identifed the ‘core genome’ shared by diverse Vitis species from North America, Europe and Asia, then used it to discover 2000 robust DNA markers covering 97% of the Vitis genome. This rhAmpSeq platform increased transferability of markers from 2% with previous SNP-based markers to 91% across the Vitis genus, including species 30 million-years diverged.
In this webinar, we will present some challenges with previous marker platforms, our approach to marker design, some exciting results, and our strategy to make this marker platform openly accessible and useful for the grape community.
Registration Link: https://cornell.zoom.us/webinar/register/WN_GitbNiqPRjGuGrIU9m6-zQ
Please email firstname.lastname@example.org with any questions.
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Funded by the USDA-NIFA Specialty Crop Research Initiative VitisGen2 project (Award No. 2017- 51181-26829)